Articles

Understanding Cuvette Volume, Material, Path Length etc.

Understanding Cuvette Volume, Material, Path Length etc. A spectrophotometer is an ideal choice for measuring materials of interest light absorbance or transmittance to a specific spectrum wavelength, in this way to get data on substance concentration and purity. A cuvette is a small vessel containing samples used for spectroscopic measurements which are placed in the spectrophotometers. Cuvettes are made of various sizes, volumes, and materials, and should be transparent for the targeted wavelength range. Types of Cuvettes For the photometric measurements of...

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Vis Cuvette Selection (not UV)

Vis Cuvette Selection (not UV) The first factor to consider when performing spectroscopy measurements is to know the range of spectrum in which you work. The selection of cuvette material is based on this range, and if you are on a tight budget, this will help to save you a lot.  To begin with, “VIS” is short for “visible” which means the visible light spectral range. The visible light spectrum is the electromagnetic spectrum portion that the human eye can see....

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Quartz Cuvette Cleaning Procedure

Quartz Cuvette Cleaning Procedure Clean cuvettes are the foundation of any photometric measurements. Even plastic cuvettes are disposable and no need to worry about cleaning, the rest of the quartz or glass cuvettes are reusable and require cleaning to avoid cross-contamination. Quartz Cuvettes Require Cleaning Hot Custom 10mm Path, 32uL, 3 Windows, NRC, FCTN42, All Black Wall Flow Through Cell, Steel Connector 0 out of 5 $223.00 /pc Select options Add to wishlist Quick View Hot Custom 10mm Path, 32uL, 2 Windows, NRC, FCTN41, All Black Wall Flow Through Cell,...

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UV vis Spectroscopy – How to Use a Spectrophotometer

UV vis Spectroscopy - How to Use a Spectrophotometer In this article we will show you how to use a UV visible spectrophotometer. Instrument: Shimadzu UV-1800 UV Spectrophotometer. These are the double beam UV visible spectrophotometers in the instrument room in the general teaching laboratory at the National University of Singapore. Take note that the on/off switch is at the right side of the spectrophotometer. Here are all the apparatus that you need for this setup: a solvent bottle, a waste beaker, a...

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UV vis Cuvette Selection: Cuvette Material and Path Length

UV vis Cuvette Selection: Cuvette Material and Path Length Selecting cuvettes for UV vis spectroscopy can be complicated if you are new to the field. Not all cuvette material will work for every experiment, so some basic instruction is required. Using this page as a guide we'll walk you through the important factors you ought to consider when buying a UV vis cuvette.  Cuvette Material Breakdown The most important factor to consider when finding a suitable cuvette is the cuvette material. There...

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UV visible Spectrophotometer Setup and Beer-Lambert Law

UV visible Spectrophotometer Setup and Beer-Lambert Law In this article we are going to have a brief discussion of UV visible spectroscopy and the Beer-Lambert law. UV vis Spectrophotometer Setup To start with our session below is an engineering design that is a relatively typical type of setup for a UV visible spectrophotometer. A variety of different brands and designs are available to manufacture a spectrophotometer but this is among the simplest ways. Let’s continue from here. Simple UV vis Spectrophotometer Setup Simple Spectrophotometer...

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