Vis Cuvette Selection (not UV)

Vis Cuvette Selection (not UV)

The first factor to consider when performing spectroscopy measurements is to know the range of spectrum in which you work. The selection of cuvette material is based on this range, and if you are on a tight budget, this will help to save you a lot. 

To begin with, “VIS” is short for “visible” which means the visible light spectral range. The visible light spectrum is the electromagnetic spectrum portion that the human eye can see. A VIS cuvette is the cuvette that works in the transmission range of about 380-700 nm [NASA]. These types of cuvettes are the cheapest among all materials that are used for making cuvettes (UV-vis Cuvettes, IR Cuvettes, UV-vis-IR Cuvettes).

visible wavelength range


What are the materials available for vis cuvettes?

Please see our article for a basic idea of the various cuvette materials we offer: UV vis Cuvette Selection: Cuvette Material and Path Length.

Please check the below table a list of cuvette materials that work under visible spectral wavelength range, including each material’s complete range of transmission. The materials are listed from price low to high.

Name Usable Range Usable Spectrum
Polystyrene VIS 340-900 nm
Acrylic VIS 285-900 nm
UV Plastic UV-VIS 220-900 nm
Optical Glass VIS 340-2,500 nm
UV Quartz UV-VIS 190-2,500 nm
IR Quartz UV-VIS-IR 250-3,500 nm

Could I use a vis cuvette for fluorescence?

Definitely! A vis cuvette will work for fluorescence measurements with four polished sides.  If your vis cuvette has two parallel sides clear and the other two frosted, it’s not gonna work.  Check a list of our in stock  fluorescence cuvettes

Standard Fluorescence Cuvettes

Some suggestions on Vis disposable cuvettes

Because of the low cost, many researchers like to choose disposable cuvettes for  Vis measurements. And the disposable cuvettes usually come in 100pcs/pkg, no need to clean and wash. Fantastic!

Here are some basic instructions on how the disposable cells can be used:

  1. Make sure that 2 – 3 cuvettes are checked when zeroing the machine to confirm their transmission are similar. The output of plastic cuvettes can differ by different batches.
  2. Make sure the optical surfaces have no scratches or imperfections, as plastic cells can scratch very easily.
  3. Keep the cover on the cuvettes whenever not in measurement to avoid contamination in the cells.
  4. Keep fingers away from the optical surfaces, always remember to hold the frosted sides.
  5. You can remove drops in the cuvette corners with a micropipette if you intend to reuse the sample.
  6. Keep in mind that plastic cuvettes are intended to use only once.

If you need further assistance with our technical information, please do not hesitate to contact us.

The Cuvet.Co Team

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